Guide for Choosing Antibodies

Get started on your path to discovery with the help of Universal Biotechnology. We provide a wide selection of antibodies that can be used in various research settings. Whether you're looking for a primary or secondary antibody, a monoclonal or polyclonal, or something in between, we have you covered. Our antibody suppliers provide the high-quality antibodies your research requires to decipher the complexities of disease pathways and cellular processes. Visit Universal Biotechnology to expand your scientific horizons into the limitless realm of antibodies.

Antibody Selection Guidelines

Make sure the antibody can be used for what you intend

An antibody's efficacy in one setting does not guarantee its performance in another. The epitope recognised by an antibody in an IHC study may not be accessible in a reduced and denatured sample used in a Western blot analysis, and vice versa. All of the uses that have been tried and tested with the antibody should be listed on the product datasheet that comes with it. Those applications in which it has created the predicted staining pattern should be mentioned, together with batch-specific statistics.

The host species and clonality must be chosen carefully

To prevent secondary antibodies from reacting with the sample's endogenous immunoglobulins, the primary antibody's host species must be different from that of the test sample's species. The clonality of the main antibody is also an important factor to consider. The advantages of monoclonal antibodies are reduced background staining, reliable experimental results, and reagent availability throughout a research project because each antibody only recognises a single antigenic epitope. Potential drawbacks include sensitivity to epitope degradation during sample treatment and inability to detect antigens across species. Polyclonal antibodies have the potential for signal amplification and antigen change tolerance due to their ability to bind many epitopes. Still, they can also be linked to undesirable background staining and batch-to-batch variability.

Select an appropriate secondary antibody

Secondary antibodies, generated against the primary antibody's host species, come in various formats from which to choose. They provide an easy way to boost a signal by binding many secondary antibodies to a single primary antibody. Still, their presence can restrict what you can do in the lab. Secondary antibody detection multiplexing, for instance, necessitates that each primary antibody be grown in a distinct host, and in some cases, directly conjugated primary antibodies may be preferred. As with all antibodies, rigorous validation is necessary to guarantee that cross-reactivity does not create misleading data when using primary antibody conjugates to increase experimental complexity and decrease the number of stages within an immunostaining technique.

Visit Universal Biotechnology to learn more about Antibodies of All Kinds

Our antibody segments are sent in top form, following thorough analysis, and with the assurance that they will improve the quality of your research and diagnostic procedures thanks to our connections with the leading antibody suppliers in India. Brands like Affinity Bioscience, GeneTex, Cloud Clone, and Elabscience are represented in our selection of antibodies. You may rest easy knowing that the content you receive will always be high quality.

We provide a comprehensive service so you can rely on us for the best antibody price in India. Universal Biotechnology strives for the best of you, and your interests maintain us at the top. We care about the results of your studies, experiments, and diagnoses using our products. Their importance in your procedures compels us to deliver only what will produce desirable outcomes.