Exploring the Working of Protein Ladders in Molecular Biology

Protein ladders occupy a significant position in molecular biology due to their enhanced functioning. These reagents comprise purified protein strains that are of familiar size and characteristics. Hence, they are therefore used to estimate the size and characteristics of unknown molecules. The speed of the molecules is inversely dependent upon their size, the more the size, the lesser the speed. To learn more about the working of protein ladders in molecular biology, start reading this blog right away!

How Do Protein Ladders Work in Molecular Biology?

Here's how protein ladders function:

  • Concept of Protein Ladders

Protein ladders are also termed protein markers, protein standards, etc. It is the most commonly used reagent in molecular biology to determine protein size using the process of gel electrophoresis. This reagent comprises purified protein stains that can be used to estimate proteins during the procedure of electrophoresis. There are various types of Protein ladders. The most commonly used ones are as follows:

  • Prestained ladders

This is one of the most common types of protein markers used in laboratories. It comes accompanied by pre-stained dyes, making it easier to determine protein migration patterns. BLUelf Prestained Protein Ladder is an effective example of prestained ladder.

  • Unstained ladders

Unstained ladders comprise purified proteins along with no synthetic dye. Since these ladders do not comprise any artificial dye, it becomes much smoother to equate the actual weight of molecular proteins as there is minimum or no interference. 

  • Western Blot ladders

The mixture of pre-stained and StrepTactin-HRP Conjugate proteins is termed a Western blot protein ladder. It makes the protein gels appear fluorescent and the western blot appear chemiluminescent.

  • Fluorescent ladders

This type of protein marker is primarily used in polyacrylamide gel electrophoresis procedures. It is an amalgamation of pre-stained proteins and fluorescent dyes. The reason behind this type of protein marker being popular is its low background interference and sensitivity.

  • Formation and Function

Protein ladders are designed to perform a wide range of functions in molecular biology and biochemistry. This popular reagent present in science labs works by determining protein sizes, including protein mechanisms and discovery. The working of protein ladders depends upon the purified protein mixtures present in them. These mixtures are present according to their original molecular weight in descending order.

The smaller molecules are responsible for performing positive electrolysis by traveling through the tiny pores of the gel Matrix and moving away from the sample loading. The bigger molecules on the other hand remain near the sample loading due to their larger size. It enables them to move through the pores. This procedure is termed negative electrolysis. 

  • Conclusion

Protein ladders undoubtedly have an immense significance in the realm of molecular biology. Are you looking for a reliable and reputed online biotechnology store to check out reagents, consumables, biotech equipment, etc.? You can take a look at Universal Biotechnology, a global biotech consultancy company providing the best products and services for a long time such as BLUeye Prestained Protein Ladder and a lot more!



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