One-Step RT-PCR Kit Vs. Two-Step RT-PCR Kit: Detailed Differences
Often individuals get confused between choosing One-Step RT-PCR Kit and Two-Step RT-PCR kit. Well, both come with their unique set of advantages and uses. In this blog, you will get to learn about both setups in a detailed description. Comparison between One-Step RT-PCR Kit and Two-Step RT-PCR Kit Here's a concrete comparison between both setups, allowing you to decide which one's more suitable for your requirements: 1. One-Step RT-PCR Kit The One-Step RT-PCR Kit consists of a single reaction tube, the first-strand cDNA synthesis (RT) and subsequent PCR. This kit setup makes the workflow much easier by greatly reducing the possibilities of variation. It also works to minimize the chances of contamination. This kit setup allows high-throughput applications and hence can be used for a large number of samples. For the amplification procedure, this kit involves using gene-specific primers. As a result, the results for gene analysis per RNA sample are limited. You wil